Table 3.
S. pombe genes identified in a screen for clotrimazole- and terbinafine-resistant genese
| Category and systematic gene name | Common gene name | Gene descriptiona | Spot assay result with: |
MIC (μg/ml)d |
||
|---|---|---|---|---|---|---|
| Clot | Terb | Clot | Terb | |||
| Wild type | 2.0 | 0.25 | ||||
| Mitochondrial function | ||||||
| SPAC23C11.08 | php3 | CCAAT binding factor complex subunit Php3 | +++ | +++ | 4.0 | 0.5 |
| SPCC1442.05cc | NA | Conserved fungal protein | ++ | + | 2.0 | 0.25 |
| SPBC27B12.10c | tom7 | Mitochondrial TOM complex subunit Tom7 | ++ | ++ | 4.0 | 0.5 |
| SPBC691.04 | mss116b | Mitochondrial ATP-dependent RNA helicase Mss116 | ++ | ++ | 2.0 | 0.5 |
| SPBC713.08 | tom13c | Mitochondrial TOM complex subunit Tom13 | − | + | 2.0 | 0.5 |
| Ubiquitination | ||||||
| SPAC15A10.11 | ubr11 | N-end-recognizing protein | ++ | + | 4.0 | 0.25 |
| Membrane trafficking | ||||||
| SPBC691.03c | apl3 | AP-2 adaptor complex subunit Alp3 | ++ | +++ | 4.0 | 0.5 |
| Cell polarity | ||||||
| SPBC15D4.10c | amo1 | Nuclear rim protein Amo1 | + | + | 2.0 | 0.5 |
| Chromatin remodeling | ||||||
| SPBP23A10.05 | ssr4c | SWI/SNF and RSC complex subunit | + | + | 2.0 | 0.5 |
| Unknown functions | ||||||
| SPBC1861.05 | NA | Carbohydrate kinase | ++ | ++ | 2.0 | 0.5 |
| SPBC106.07c | nat2b | N-alpha-acetylation-related protein | + | ++ | 2.0 | 0.5 |
a
Gene description as indicated in the Sanger Center S. pombe database. TOM, translocase of outer membrane; RSC, remodel the structure of chromatin.
b
The common name is taken from the orthology of S. cerevisiae.
c
The gene is conserved in fungi only.
d
Values obtained by using the MIC-2 (50% growth inhibition) endpoint.
e
+++, strongly resistant; ++, moderately resistant; +, mildly resistant; −, not resistant; NA, the common gene name is not applicable.