Fig 4.

Effect of time of treatment with 13.75 mM EIP-K plus 3 mM H₂O₂ on bactericidal activity (A) and DNA condensation (B an C) in E. coli MC4100. The values are means and standard errors of the mean for three experiments, each run in duplicate or triplicate. (A) There is a significant effect of treatment time with EIP-K plus H₂O₂ on the number of CFU (one-way ANOVA, F_[7,8] = 83.86; P < 0.0001), and post hoc Scheffé tests (α = 0.05) show that the untreated group (time = 0) is significantly different than all treated groups. (B and C) The negative control, “control 10 min,” is 10-min exposure to ddH₂O, and the number of cells (n) for each treatment is shown in the bars. One-way ANOVA for nucleoid length (B) shows a significant effect of treatment time (F_[5,2433] = 320.35; P < 0.001), and post hoc Scheffé tests (α = 0.05) show that the control group is significantly different than the other groups, as indicated by asterisks. One-way ANOVA for form factor (C) shows a significant effect of treatment time (F_[5,2433] = 394.99; P < 0.001), and post hoc Scheffé tests (α = 0.05) show that the control group has a nucleoid that is significantly rounder than the other groups, as indicated by asterisks.