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Structure-activity bioassay experiments characterizing the efficacy of VanJ. Paper discs containing approximately 30 μg/ml of each glycopeptide shown were placed on freshly spread lawns of a ΔvanRS indicator strain carrying the ermEp-vanJ overexpression plasmid pGN024 (+, strain H243) or only the empty vector (−, strain H102). Plates are shown after 2 days of incubation at 30°C.
