Fig 7.

(A) Amino acid sequence alignment of VanJ and StaP. The annotated translational start sites of both VanJ and StaP are marked with arrowheads, and the alternative translational start sites in the StaP sequence are indicated by arrows. The black bar beneath the amino acid sequence alignment represents the conserved pfam03372 endonuclease/exonuclease/phosphatase domain. (B) StaP is an orthologue of VanJ. Constitutive expression of the annotated staP gene (pGN026) in S. coelicolor has no effect on teicoplanin resistance, but constitutive expression of staP encoding all four potential translational start codons (pGN027) increased resistance to teicoplanin, analogous to the results for VanJ shown in Fig. 2A. MIC values are indicated beneath the bioassay plates, and the staP gene inserts present in pGN026 and pGN027 are illustrated on the right.