Plasmids |
|
|
pMS81 |
ϕBT1 attP-int-derived integration vector for the conjugal transfer of DNA from E. coli to Streptomyces spp. (Hygr) |
17 |
pIJ10257 |
330-bp ermEp (KpnI-PstI) with ribosome binding site and multicloning site from pIJ8723 cloned into pMS81 cut with KpnI-NsiI (Hygr) |
24 |
pIJ790 |
Modified λRED recombination plasmid pKD20 |
18 |
pGN030 |
pMS81 carrying vanJ with its own promoter sequence (p-vanJ) |
This study |
pGN037 |
pMS81 carrying p-vanJ with a C-terminal 6-histidine tag (C-6His tag); a stop codon (UGA) was introduced in frame immediately before the annotated start codon |
This study |
pGN038 |
pMS81 carrying vanJp with a C-6His tag |
This study |
pGN034 |
Short vanJ (annotated vanJ sequence) with a C-6His tag cloned in pIJ10257 under ermEp control |
This study |
pGN035 |
Long vanJ (including an extra 117 bp at the 5′ end) with a C-6His tag cloned in pIJ10257 under ermEp control |
This study |
pDU001 |
vanS cloned in pIJ10257 under ermEp control |
Novotna and Hong (unpublished) |
pGN091 |
vanS with a C-6His tag cloned in pIJ10257 under ermEp control |
This study |
pGN024 |
Long vanJ cloned in pIJ10257 under ermEp control |
This study |
pGN026 |
Short staP (annotated staP sequence) cloned in pIJ10257 under ermEp control |
This study |
pGN027 |
Long staP (including an extra 246 bp at the 5′ end) cloned in pIJ10257 under ermEp control |
This study |
pKV33 |
SCO2255 gene cloned in pIJ10257 under ermEp control |
This study |
pKV35 |
SCO7017 gene cloned in pIJ10257 under ermEp control |
This study |
pCH32 |
SAV5946 gene cloned in pIJ10257 under ermEp control |
This study |
Streptomyces strains |
|
|
S. coelicolor M600 |
SCP1− SCP2−
|
29 |
S. coelicolor H27 |
M600 + pMS81 |
This study |
S. coelicolor H2077 |
M600 + pIJ10257 |
This study |
S. coelicolor H2050 |
M600 + pGN024 |
This study |
S. coelicolor H357 |
M600 + pKV33 |
This study |
S. coelicolor H358 |
M600 + pKV35 |
This study |
S. coelicolor H359 |
M600 + pCH32 |
This study |
S. coelicolor H2141 |
M600 + pGN026 |
This study |
S. coelicolor H2148 |
M600 + pGN027 |
This study |
S. coelicolor J3201 |
ΔvanRS SCP1− SCP2−
|
25 |
S. coelicolor H102 |
J3201 + pIJ10257 |
This study |
S. coelicolor H243 |
J3201 + pGN024 |
This study |
S. coelioclor H2135 |
J3201 + pGN026 |
This study |
S. coelicolor H2143 |
J3201 + pGN027 |
This study |
S. coelicolor J3220 |
ΔvanJ::apr SCP1− SCP2−
|
23 |
S. coelicolor H101 |
J3220 + pMS81 |
This study |
S. coelicolor H215 |
J3220 + pIJ10257 |
This study |
S. coelicolor H291 |
J3220 + pGN030 |
This study |
S. coelicolor H2389 |
J3220 + pGN038 |
This study |
S. coelicolor H2391 |
J3220 + pGN037 |
This study |
S. coelicolor H2087 |
J3220 + pGN024 |
This study |
S. coelicolor H2280 |
J3220 + pGN034 |
This study |
S. coelicolor H2281 |
J3220 + pGN035 |
This study |
S. coelicolor H2137 |
J3220 + pGN026 |
This study |
S. coelicolor H2145 |
J3220 + pGN027 |
This study |
S. coelicolor J3200 |
ΔvanS SCP1− SCP2−
|
25 |
S. coelicolor H2276 |
J3200 + pGN091 |
This study |
S. coelicolor J3226 |
ΔvanHAX::apr SCP1− SCP2−
|
23 |
S. coelicolor H2270 |
J3226 + pIJ10257 |
This study |
S. coelicolor H256 |
J3226 + pGN024 |
This study |
S. coelicolor H2139 |
J3226 + pGN026 |
This study |
S. coelicolor H2147 |
J3226 + pGN027 |
This study |
S. coelicolor H296 |
ΔSCO2255::apr SCP1− SCP2−
|
This study |
S. coelicolor H347 |
ΔSCO7017::apr SCP1− SCP2−
|
This study |
S. toyocaensis NRRL15009 |
A47934 producer; wild type |
37 |
S. avermitilis ATCC 31267 |
Avermectin producer; wild type |
44 |
S. avermitilis H334 |
ΔSAV5946::apr
|
This study |
E. coli strains |
|
|
ET12567(pUZ8002) |
ET12567 containing helper plasmid pUZ8002 |
35 |
BW25113(pIJ790) |
BW25113 containing helper plasmid pIJ790 |
18 |