Table 2.
Name of oligonucleotide | Nucleotide sequence (5′–3′)a |
---|---|
vanJ-L stop c113g anti | CCACCCGTCACAGCCGTCTCCGAG |
vanJ-L stop c113 | CTCGGAGACGGCTGTGACGGGTGG |
pJsc EcoNI IF F | CGCGGATCGCCTCCGCCAGGTAGGGCTCGTCCTCGAC |
pJsc C-6HIS HindIII IF R | CCTAGGATCCAAGCTTTCA GTGGTGGTGGTGGTGGTGCCAGCTGACCCCCGCCGCCAC |
vanJsc C-tag Xmnl F | AGGCGGCCGGGAACGGGTTCGGCTTCACCTGGCCGGCGAAG |
vanJsc C-6His HindIII R | CCTAGGATCCAAGCTTTCAGTGGTGGTGGTGGTGGTGCCAGCTGACCCCCGCCGC |
vanSsc C-tag StuI F | TCAGCCCCCACCAGGCCTCGACCCTCACCGAACCCTTC |
vanSsc C-6His HindIII R | CCTAGGATCCAAGCTTTCAGTGGTGGTGGTGGTGGTGCCTGCCGGTGTGCGGAGC |
LvanJ-I | CATATGCGTCAGACCTCACCACCA |
vanJ II | CTCGAGTCACCAGCTGACCCCCGCCGC |
vanJst I | CATATGGAGACCTTCCTGCCGTG |
vanJst-L I | CATATGATCAATGAGCACCTGCG |
vanJst II | GATATCTTAATTAATCACCAGCTGATTCGGGCCG |
2255 KO I | GGCGACGGTGTGCACAGCAGGCAGTGAGGCAACGGTATGATTCCGGGGATCCGTCGACC |
2255 KO II | CAAAGACTCTCAGGGCGGGTATTCCGCAGACGAATTCTATGTAGGCTGGAGCTGCTTC |
7017 KO I | CCGGGTCGGGCGAGCTGGACGCGCGGGCGCGGACGGGTGATTCCGGGGATCCGTCGACC |
7017 KO II | ACGAAAGGCTAACGCAAACACCGCAAGCGGCTTGCTTCATGTAGGCTGGAGCTGCTTA |
SCO2255 FLANK F | CATATGGCGCAGCAGGCGTACAT |
SCO2255 FLANK R | TTAATTAAGACGAATTCTAGGAAGCCG |
SCO7017L FLANK F | CATATGGTGGAGCGGGTGGGCCGT |
SCO7017 FLANK R | TTAATTAAGGCTTGCTTCAGTCCCAGG |
SCO2255 TEST F | CGGCACAGTGCGGAACTCC |
SCO2255 TEST R | GGTCTCGCCTTTCGAGAGC |
SCO7017 TEST F | TTCGGTGGTGAAGACGACG |
SCO7017 TEST R | CCCCGGTTTGCCGTTGAGC |
SAV_5946 KO F | AGTGACGGTGTGCACGGCAGGTTGTGAGGCGACGGTATGTGTAGGCTG GACCTGCTTC |
SAV_5946 KO R | ACGGAACAAATAGGCTCGGGCCGAGTATTCCACGAGCTAATTCCGGGGATCCGTCGACC |
SAV_5946 TEST F | ACCACTAATGTGCGCGTTTC |
SAV_5946 TEST R | AGAGGCCTTTCACGAGCAAG |
SAV_5946 pIJ10257 F | AATTCATATGGCGCAGGCGTACGTGAC |
SAV_5946 pIJ10257 R | AATTAAGCTTCTAGGAAGCAGTTGCGGAG |
Restriction sites used for cloning are underlined.