Table 2.
Oligonucleotides used in this study
| Name of oligonucleotide | Nucleotide sequence (5′–3′)a |
|---|---|
| vanJ-L stop c113g anti | CCACCCGTCACAGCCGTCTCCGAG |
| vanJ-L stop c113 | CTCGGAGACGGCTGTGACGGGTGG |
| pJsc EcoNI IF F | CGCGGATCGCCTCCGCCAGGTAGGGCTCGTCCTCGAC |
| pJsc C-6HIS HindIII IF R | CCTAGGATCCAAGCTTTCA GTGGTGGTGGTGGTGGTGCCAGCTGACCCCCGCCGCCAC |
| vanJsc C-tag Xmnl F | AGGCGGCCGGGAACGGGTTCGGCTTCACCTGGCCGGCGAAG |
| vanJsc C-6His HindIII R | CCTAGGATCCAAGCTTTCAGTGGTGGTGGTGGTGGTGCCAGCTGACCCCCGCCGC |
| vanSsc C-tag StuI F | TCAGCCCCCACCAGGCCTCGACCCTCACCGAACCCTTC |
| vanSsc C-6His HindIII R | CCTAGGATCCAAGCTTTCAGTGGTGGTGGTGGTGGTGCCTGCCGGTGTGCGGAGC |
| LvanJ-I | CATATGCGTCAGACCTCACCACCA |
| vanJ II | CTCGAGTCACCAGCTGACCCCCGCCGC |
| vanJst I | CATATGGAGACCTTCCTGCCGTG |
| vanJst-L I | CATATGATCAATGAGCACCTGCG |
| vanJst II | GATATCTTAATTAATCACCAGCTGATTCGGGCCG |
| 2255 KO I | GGCGACGGTGTGCACAGCAGGCAGTGAGGCAACGGTATGATTCCGGGGATCCGTCGACC |
| 2255 KO II | CAAAGACTCTCAGGGCGGGTATTCCGCAGACGAATTCTATGTAGGCTGGAGCTGCTTC |
| 7017 KO I | CCGGGTCGGGCGAGCTGGACGCGCGGGCGCGGACGGGTGATTCCGGGGATCCGTCGACC |
| 7017 KO II | ACGAAAGGCTAACGCAAACACCGCAAGCGGCTTGCTTCATGTAGGCTGGAGCTGCTTA |
| SCO2255 FLANK F | CATATGGCGCAGCAGGCGTACAT |
| SCO2255 FLANK R | TTAATTAAGACGAATTCTAGGAAGCCG |
| SCO7017L FLANK F | CATATGGTGGAGCGGGTGGGCCGT |
| SCO7017 FLANK R | TTAATTAAGGCTTGCTTCAGTCCCAGG |
| SCO2255 TEST F | CGGCACAGTGCGGAACTCC |
| SCO2255 TEST R | GGTCTCGCCTTTCGAGAGC |
| SCO7017 TEST F | TTCGGTGGTGAAGACGACG |
| SCO7017 TEST R | CCCCGGTTTGCCGTTGAGC |
| SAV_5946 KO F | AGTGACGGTGTGCACGGCAGGTTGTGAGGCGACGGTATGTGTAGGCTG GACCTGCTTC |
| SAV_5946 KO R | ACGGAACAAATAGGCTCGGGCCGAGTATTCCACGAGCTAATTCCGGGGATCCGTCGACC |
| SAV_5946 TEST F | ACCACTAATGTGCGCGTTTC |
| SAV_5946 TEST R | AGAGGCCTTTCACGAGCAAG |
| SAV_5946 pIJ10257 F | AATTCATATGGCGCAGGCGTACGTGAC |
| SAV_5946 pIJ10257 R | AATTAAGCTTCTAGGAAGCAGTTGCGGAG |
a
Restriction sites used for cloning are underlined.