Table 2.
Influence of NCT and heparin on coagulation parameters in whole human blooda
| Anticoagulant | PT (%) | INR | aPTT (s) | TT (s) | Fib (mg/dl) | AT III (%) | D dimer (μg/liter) |
|---|---|---|---|---|---|---|---|
| Control | 84.1 (10.5) | 1.1 (0.1) | 33.5 (3.9) | 19.5 (1.6) | 246.5 (54.1) | 87.5 (7.7) | 185.8 (72.1) |
| Heparin | 70.8 (11.3) | 1.2 (0.1) | 247.4b (87.2) | ≥400b (NC) | 253.1 (67.3) | 88.1 (11.0) | 193.5 (74.2) |
| 0.55 mM NCT | 74.8 (11.7) | 1.2 (0.1) | 37.6 (4.4) | 23.5 (2.9) | 192.1 (51.6) | 86.2 (8.1) | 179.9 (72.6) |
| 1.38 mM NCT | 25.3b,c (11.1) | 3.2b,c (1.3) | 114.0d (53.6) | 45.8b,c (7.2) | ≤40b,c (NC) | 83.7 (8.3) | 165.5 (68.1) |
| 0.55 mM NCT + heparin | 62.5b (10.5) | 1.3 (0.1) | 295.5b (80.2) | ≥400b (NC) | 192.5 (50.7) | 84.5 (9.0) | 184.6 (73.7) |
NCT was dissolved in saline and 10-fold diluted in whole human citrate blood (0.2 ml plus 1.8 ml) with or without heparin (0.5 IU/ml) to 0.55 mM (0.01%) or 1.38 mM (0.025%). Control consisted of 0.2 ml 0.9% NaCl plus 1.8 ml whole citrate blood. NC, not calculable; PT, prothrombin time; INR, international normalized ratio; aPTT, activated partial thromboplastin time; TT, thrombin time; Fib, fibrinogen; AT III, antithrombin III; D dimer, cleavage products of polymerized fibrin. Values are means (standard deviations) of 3 to 4 independent experiments.
P < 0.01 versus control (one-way ANOVA and Bonferroni's multiple-comparison test).
P < 0.01 versus all other test rows (one-way ANOVA and Bonferroni's multiple-comparison test).
P < 0.05 versus control (one-way ANOVA and Bonferroni's multiple-comparison test).