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F2 inhibits the ClpXP protease. (A) Structure of F2; (B) pulse-chase assays to monitor proteolysis of the ClpXP substrate protein GFP-AA in the presence or absence of 100 μM F2; (inset) a representative blot. The graph depicts quantified band intensity of GFP, where the value for wild-type bacteria without F2 is set at 100% and mean results ± standard deviation from 3 experiments are shown.
