Fig 2.

Cysteine residues in the zinc finger motif are critical for DksA-dependent antinitrosative defense. Wild-type (WT), ΔdksA, or isogenic ΔdksA Salmonella strains complemented with wild-type DksA (cDksA) or a DksA C114S, C117S, C135S, or C138S variant were treated with 5 mM DETA (control) (A) or DETA NONOate (dNO) (B) as described in the legend for Fig. 1. Bacterial growth is expressed as the OD₆₀₀ over time, and the data are represented as the means of 10 independent observations from 2 separate experiments. The P value for the difference between the ΔdksA and isogenic ΔdksA Salmonella strains complemented with DksA C114S, C117S, C135S, or C138S and the wild-type strain was <0.0001 as determined by one-way ANOVA of all data in the curve followed by Bonferroni's posttest.