Fig 5.

Role of DksA in the intracellular survival of Salmonella in iNOS-expressing macrophages. The antimicrobial activities of periodate-elicited peritoneal macrophages isolated from C57BL/6 mice (A) or congenic iNOS-deficient controls (B) were evaluated over time after mice were challenged with the wild-type (WT) or the ΔdksA mutant Salmonella strain. The number of surviving intracellular bacteria was estimated after culture on LB agar plates as described in Materials and Methods. The data represent the mean percent survival ± the SEM of four to eight independent observations from at least two independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001 compared to results for WT controls.