Table 2.
Primers used for the construction of strains used in this study
| Mutation | Primer sequencea (5′–3′) |
|---|---|
| dksAC114S | F: TGGAAGATGAAGACTTCGGTTATAGCGAGTCCTGCG |
| R: CGCAGGACTCGCTATAACCGAAGTCTTCATCTTCCA | |
| dksAC117S | F: TATTGCGAGTCCAGCGGGGTGGAGATT |
| R: AATCTCCACCCCGCTGGACTCGCAATA | |
| dksAC135S | F: ACAGCCGATCTGAGCATCGACTGCAAAACGCTGGCT |
| R: AGCCAGCGTTTTGCAGTCGATGCTCAGATCGGCTGT | |
| dksAC138S | F: ACAGCCGATCTGTGCATCGACAGCAAAACGCTGGCT |
| R: AGCCAGCGTTTTGCTGTCGATGCACAGATCGGCTGT | |
| pSK::cm | F: ACGCGGATCCATGGGAATTAGCCATGGTCC |
| R: CTGCAGGAGCTCGTGTAGGCTGGAGCTGCTTC | |
| pSK::dksA::cm | F: ATCGTAGAATTCCGTTGTAGTGGAATAACAGC |
| R: CCGCGGATCCTTAACCCGCCATCTGTTTTT | |
| put::dksA | F: TAGCGATGGGAGAGAGGACACGTTAATTATTCCATTTTAA |
| TAGTGGAATAACAGCCTGATTATTA | |
| R: TACTGCGGGTATTAATGCTGAAAACATCCATAACCCATTGGTGT | |
| AGGCTGGAGCTGCTTC |
a
Underlined sequences denote SacI, BamHI, or EcoRI restriction sites.