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. 2012 Apr;80(4):1373–1380. doi: 10.1128/IAI.06316-11

Table 2.

Primers used for the construction of strains used in this study

Mutation Primer sequencea (5′–3′)
dksAC114S F: TGGAAGATGAAGACTTCGGTTATAGCGAGTCCTGCG
R: CGCAGGACTCGCTATAACCGAAGTCTTCATCTTCCA
dksAC117S F: TATTGCGAGTCCAGCGGGGTGGAGATT
R: AATCTCCACCCCGCTGGACTCGCAATA
dksAC135S F: ACAGCCGATCTGAGCATCGACTGCAAAACGCTGGCT
R: AGCCAGCGTTTTGCAGTCGATGCTCAGATCGGCTGT
dksAC138S F: ACAGCCGATCTGTGCATCGACAGCAAAACGCTGGCT
R: AGCCAGCGTTTTGCTGTCGATGCACAGATCGGCTGT
pSK::cm F: ACGCGGATCCATGGGAATTAGCCATGGTCC
R: CTGCAGGAGCTCGTGTAGGCTGGAGCTGCTTC
pSK::dksA::cm F: ATCGTAGAATTCCGTTGTAGTGGAATAACAGC
R: CCGCGGATCCTTAACCCGCCATCTGTTTTT
put::dksA F: TAGCGATGGGAGAGAGGACACGTTAATTATTCCATTTTAA
TAGTGGAATAACAGCCTGATTATTA
R: TACTGCGGGTATTAATGCTGAAAACATCCATAACCCATTGGTGT
AGGCTGGAGCTGCTTC
a

Underlined sequences denote SacI, BamHI, or EcoRI restriction sites.