Fig 3.
Association of cell cycle state in J774.16 macrophages with fungal burden. Macrophage progression to the G2/M phase of cell cycle correlated with fungal burden within the macrophage. Macrophages were grouped according to the number of yeasts ingested and their cell cycle phases. (A and B) Percentage of macrophages in the G1 phase (A) and G2/M phase (B). Experiments were performed three times, with six replicate wells. The data points and averages are shown. (C) Percentage of EdU+ macrophages during phagocytosis. Macrophages were allowed to incorporate EdU for 2 h at an MOI (macrophage/yeast ratio) of 0 (no yeasts), 1:2, or 1:10. The experiment was repeated twice in duplicate wells with similar results. Shown are means ± the SD of duplicate wells of a representative experiment.