Fig 4.

EdU incorporation and J774.16 macrophage completion of cycle. Ingestion of C. neoformans decreased the number of macrophages that successfully divided and returned to the G₁ phase. These macrophages were retained at the G₂/M phase. Macrophages that had not ingested C. neoformans (No Cn) after 2 h of C. neoformans phagocytosis were allowed to incorporate EdU for 6 h. (A) Percentage of total macrophages that incorporated EdU. (B) Percentage of macrophages that incorporated EdU, underwent mitotic division and subsequently reached the G₁ phase. (C) Percentage of macrophages that incorporated EdU and reached the G₂/M phase. Experiments were performed three times. Shown are means ± the SD of triplicate wells of a representative experiment. Groups were compared using a two-tailed t test with a 95% confidence interval (*, P < 0.05; n.s., not significant).