Fig 6.

Y. pestis-induced apoptosis of J774.1 macrophages can be prevented with anti-rV10 or MAb-BA5 antibodies. Y. enterocolitica W22703, Y. enterocolitica WA-314, Y. pestis KIM D27, Y. pestis NCM3, and Y. pestis NCM4 were used to infect J774.1 macrophages at an MOI of 10 in the absence or presence of 1 mg of anti-rV10 or MAb-BA5 antibody. Samples were stained with propidium iodide to identify and enumerate apoptotic remnants of macrophages. The data from at least three independent determinations with more than 1,000 cells counted for each condition were averaged, and the standard errors of the means were calculated. Control conditions (naive serum and PBS) were compared individually to each specific test condition. The statistical significance was calculated using two-tailed unpaired Student's t test comparing PBS (mock) or naive serum samples with anti-rV10 or MAb-BA5 (*, P < 0.05; **, P < 0.01).