Fig 1.

Leukocytosis and colonization of pertussis-infected rhesus macaques. Four weanling rhesus macaques were inoculated with B. pertussis strain D420 as described in Materials and Methods. Once prior to inoculation and on the indicated days postinoculation, blood was drawn from each rhesus macaque, and the number of circulating white blood cells per microliter of peripheral blood was determined as described in Materials and Methods and shown in the graph for each animal. Nasopharyngeal washes were performed as described in Materials and Methods, and the recovered washes were plated on two Regan-Lowe plates (50 μl per plate). The number of CFU per plate is indicated for each day by the shading of the bar over the WBC graph: white, 0 colonies; light gray, 1 to 100 colonies; dark gray, 101 to 1,000 colonies; and black, >1,000 colonies.