Fig 2.

Immunization of mice with AnAPN1-Alhydrogel elicited a potent antigen-specific humoral response and functional, malaria parasite transmission-blocking antibodies. Sera were collected from the satellite mouse control cohort C (a) and APN cohort D (b). Only sera from cohort D demonstrated an AnAPN1-specific antibody response. Mice were immunized following the prime–three-boost schedule (5 μg/dose) used for mouse cohorts A and B, which were subsequently analyzed for gross and subpatent histopathologies. (c) Representative results from passive immunization feeding assays (PIFA) that demonstrate the potent transmission-blocking activity of AnAPN1-specific IgG (100 μg/ml) collected from mice in cohorts B and D against Plasmodium berghei in Anopheles stephensi mosquitoes. We observed reductions in median oocyst infection intensity between 80 to 100%. Each set of pre- and postexperiment values represents a single biological replicate. *, P < 0.05; **, P < 0.01.