Table 3.
Quantification of cytokine expression in RAJ tissues at day 28 by QRT-PCR
| Cytokine/chemokine or transcription factor and E. coli strain | Fold changea | P valueb |
|---|---|---|
| IL-1β | ||
| Wild type | 0.692 | |
| hha | 0.596 | NSc |
| hha sepB | 2.340 | <0.01 |
| IL-8 | ||
| Wild type | 8.505 | |
| hha | 6.870 | NS |
| hha sepB | 9.636 | NS |
| IL-10 | ||
| Wild type | 0.927 | NS |
| hha | 0.542 | NS |
| hha sepB | 0.307 | NS |
| IL-13 | ||
| Wild type | 0.055 | |
| hha | 0.035 | NS |
| hha sepB | 0.883 | NS |
| IL-17 | ||
| Wild type | 0.625 | |
| hha | 0.294 | NS |
| hha sepB | 0.144 | NS |
| IL-22 | ||
| Wild type | 1.088 | |
| hha | 0.960 | NS |
| hha sepB | 0.195 | NS |
| TNF-α | ||
| Wild type | 0.821 | |
| hha | 0.32 | NS |
| hha sepB | 0.287 | NS |
| T-bet | ||
| Wild type | 0.615 | |
| hha | 0.355 | NS |
| hha sepB | 0.191 | NS |
| GATA3 | ||
| Wild type | 6.06 | |
| hha | 10.016 | NS |
| hha sepB | 4.668 | NS |
| RORγT | ||
| Wild type | 4.629 | |
| hha | 3.555 | NS |
| hha sepB | 2.447 | NS |
a
Fold change (calculated as 2−ΔΔCT) in RAJ tissue cytokine gene expression relative to small intestinal tissue using the bovine ribosomal protein S9 coding gene as an endogenous control. The observed variability in the ratios of target genes to RS9 among the three treatment groups was approximately 5%.
b
The level of significance of the fold change in cytokine/chemokine or transcription factor gene expression for E. coli mutants compared to the wild type was determined by ANOVA using Bonferroni's post hoc test.
c
NS, not significant.