Table 2.
Characteristics of the ORFs of the Pacdt gene and its flanking regions
| ORFa | Gene coordinates and direction | Gene product size (aa) | GC% | Related bacterial proteins |
Source or reference | ||
|---|---|---|---|---|---|---|---|
| Product(s) and origin | GenBank accession no. | BLAST E-value (identity), % | |||||
| 1 | 651←1001 | 117 | 47.86 | Transposase (fragment), Xenorhabdus nematophila ATCC 19061 | FN667742 | 4e–19 (64/110), 58% | Direct submission |
| 2 | 745→885 | 47 | 49.65 | Transposase (fragment), Xenorhabdus nematophila ATCC 19061 | FN667742 | 7e–05 (22/39), 56% | Direct submission |
| 3 | 882→1004 | 41 | 46.34 | No hits found | |||
| 4 | 1270→1368 | 33 | 36.36 | Unknown, Comamonas testosteroni PtL5 | AF076997 | 0.24 (15/22), 68% | Direct submission |
| 5 | 1387→1947 | 187 | 34.22 | Unknown, Photorhabdus luminescens subsp. laumondii TTO1 | BX571866 | 8e–65 (126/187), 67% | 53 |
| 6 | 1812←1898 | 29 | 42.53 | Putative DNA-binding protein, Proteus mirabilis HI4320 | AM942759 | 8e–06 (24/28), 85% | 42 |
| 7 | 1996←2574 | 193 | 43.01 | Transposase, Salmonella enterica subsp. salamae serovar Sofia | FJ496648 | 7e–86 (154/192), 80% | Direct submission |
| 8 | 2957←3445 | 163 | 47.65 | Putative exported protein, Citrobacter rodentium ICC168 | FN543503 | 1e–27 (55/100), 55% | 44 |
| 9 | 3464←3655 | 64 | 44.27 | Transposase, Proteus mirabilis HI4320 | AM942759 | 5e–24 (54/67), 80% | 42 |
| 10 | 3604←3801 | 66 | 41.92 | Putative putative IS element transposase, Proteus mirabilis HI4320 | AM942759 | 3e–10 (23/32), 71% | 42 |
| 11* | 4264→5013 | 750 | 42.80 | Cytolethal distending toxin A, Shigella boydii strain K-1 | AY696753 | 92% | 25 |
| 12* | 5034→5843 | 810 | 42.10 | Cytolethal distending toxin B, Shigella boydii strain K-1 | AY696753 | 95% | 25 |
| 13* | 5858→6406 | 549 | 37.34 | Cytolethal distending toxin C, Shigella boydii strain K-1 | AY696753 | 90% | 25 |
| 14 | 6884→7090 | 69 | 29.47 | Replication initiator and transcription repressor, Pantoea stewartii subsp. stewartii | L42524 | 3e–16 (47/94), 50% | 19 |
| 15 | 7390→7542 | 51 | 41.83 | Unknown, Escherichia coli strain CB853 | FM210347 | 0.044 (22/50), 44% | 10 |
| 16 | 7539→8168 | 210 | 45.40 | Hypothetical protein, Enterobacter cloacae | AY780889 | 4e–78 (142/205), 69% | 59 |
| 17 | 8168→8281 | 38 | 51.75 | Transposase, Escherichia coli strain BEN2908 | AY857617 | 1e–09 (30/41), 73% | 14 |
| 18 | 8186←8323 | 46 | 44.93 | Hypothetical ORF in IS2, Klebsiella pneumoniae | AY378100 | 0.020 (24/47), 51% | 12 |
| 19 | 8384←8542 | 53 | 35.85 | Transposase, Clostridium difficile CD196 | FN538970 | 1.7 (15/34), 44% | 49 |
| 20 | 8629←9567 | 313 | 37.49 | EspG protein, Escherichia coli strain 71074 | GQ338312 | 2e–44 (104/314), 33% | Direct submission |
| 21 | 8960→9190 | 77 | 37.66 | Pyridine nucleotide-disulfide oxidoreductase, Listeria monocytogenes HCC23 | CP001175 | 32.7 (17/50), 34% | Direct submission |
| 22 | 9861←11549 | 563 | 38.90 | Protein tyrosine phosphatase SptP, Salmonella enterica subsp. enterica serovar Typhimurium strain SL1344 | U63293 | 9e–82 (190/559), 33% | 30 |
| 23 | 11579←1195 | 124 | 31.45 | Chaperone protein SicP, Salmonella enterica subsp. salamae serovar Sofia | FJ496648 | 2e–16 (43/93), 46% | Direct submission |
a
*, Results obtained from the CLUSTAL W method.