Table 4.
Cytotoxicity of EcCDT-1 and PaCDT on various cell lines
| Strain and sample | CD50a |
|||||||
|---|---|---|---|---|---|---|---|---|
| CHO | HeLa | Vero | HEp2 | Int407 | Caco-2 | Y-1 | NIH/3T3 | |
| E. coli | ||||||||
| GB1371 (EcCDT-I) | 64 (70) | 128 (140) | 32 (35) | 16 (18) | 16 (18) | 128 (140) | <1 (<1) | <1 (<1) |
| TAS-2 (rPaCDT) | 8 (7.7) | <1 (<0.97) | 1 (0.97) | <1 (<0.97) | <1 (<0.97) | 4 (3.9) | <1 (<0.97) | <1 (<0.97) |
| P. alcalifaciens | ||||||||
| AH-31 (PaCDT) | 2 (2.2) | <1 (<1.1) | <1 (<1.1) | <1 (<1.1) | <1 (<1.1) | 1 (1.1) | <1 (<1.1) | <1 (<1.1) |
| AS-1 (PaCDT) | 8 (12) | <1 (<1.5) | 1 (1.5) | <1 (<1.5) | <1 (<1.5) | 4 (6.0) | <1 (<1.5) | <1 (<1.5) |
a
The values indicate titers as the 50% cytotoxic dose (CD50). Numbers in parentheses indicate the titer per mg of protein of the sample (n = 3).