Fig 2.

Effects of T. gondii infection on DC activation in IL-1Ra-deficient mice. (A) CD11c⁺ cells were isolated from the mLN or spleens of IL-1Ra-deficient mice with or without T. gondii infection at 1 week p.i., and mRNA expression of IL-12p40 from CD11c⁺ cells of uninfected (white column) or T. gondii-infected (black column) IL-1Ra-deficient mice was evaluated by reverse transcription-PCR as described in Materials and Methods. IL-10 mRNA (B), IL-6 mRNA (C), and IL-1β mRNA (D) expression of CD11c⁺ cells was similarly measured. The results are normalized with those for GAPDH in the same sample. Values represent the results from three independent experiments with three to five mice in each experimental group. Statistical differences by Student's t test between groups are shown as follows: *, P was <0.05, and ***, P was <0.005.