Fig 3.

Effect of T. gondii infection on Th cell polarization in IL-1Ra-deficient mice. CD4⁺ cells were isolated from either the mLN or spleens of IL-1Ra-deficient mice with or without T. gondii infection at 1 week p.i., and levels of mRNA expression of IFN-γ (A), IL-4 (B), and IL-17 (C) were comparatively measured as described in Materials and Methods. The results from uninfected mice (white columns) or T. gondii-infected mice (black columns) are normalized with GAPDH in the same sample. Values represent results from three independent experiments with three to five mice per group. Statistical differences between groups are shown as follows: *, P was <0.05; **, P was <0.01; and ***, P was <0.005.