Fig 4.

Regulation of transcription factors for Th cell polarization by T. gondii infection. CD4⁺ cells were isolated from either the mLN or spleens of IL-1Ra-deficient mice with or without T. gondii infection at 1 week p.i., and levels of mRNA expression of T-bet (A), GATA-3 (B), and RORγt (C) were comparatively measured as described in Materials and Methods. The results from uninfected mice (white columns) or T. gondii-infected ice (black columns) are normalized with GAPDH mRNA in the same sample. Values represent results from three independent experiments with three to five mice per group. Statistical differences between groups are shown as follows: *, P was <0.05; **, P was <0.01; and ***, P was <0.005.