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Msh-pilin immunoblotting of whole-cell proteins of the wild-type strain (WT) and isogenic mutants. The mutation is indicated above the lane. (A) MshA Western immunoblot; (B) MshB Western immunoblot. Proteins were obtained from bacteria grown at 37°C in BHIB and were analyzed by SDS-PAGE (12%). They were transferred onto nitrocellulose membranes and immunoblotted with anti-MshA or anti-MshB antibody (1:1,000).
