Table 1.
Bacterial strains and plasmids used in this study
| Strain/plasmid | Genotype/phenotypea | Source/reference |
|---|---|---|
| Strains | ||
| Aeromonas veronii bv. Sobria | ||
| BC88 | Wild type, dysenteric isolate | 13 |
| BC88R | BC88, spontaneous Rifr | This study |
| BC88mshA | BC88RmshA::Kmr | This study |
| BC88mshB | BC88RmshB::Kmr | This study |
| BC88mshC | BC88RmshC::Kmr | This study |
| BC88mshD | BC88RmshD::Kmr | This study |
| BC88TapD | BC88RmshD::Kmr | This study |
| BC88MshH | BC88RmshH::Kmr | This study |
| Escherichia coli | ||
| CC118λpir | Δ(ara,leu)7697 araD139 ΔlazX74 glaE glaK phoA20 thi-1 rspE rpoB(Rfr) argE(am) recA1 λpir+ | 8 |
| S17-1λpir | hsdR pro recA, RP4-2 in chromosome, Km::Tn7 (Tc::Mu) λpir Tpr Smr | 19 |
| XL1-Blue | endA1 recA1 hsdR17 supE44 thi-1 gyrA96 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tcr)] | Stratagene |
| BL21(DE3) | F−ompT gal dcm lon hsdSB (rB−mB−) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) | Novagen |
| Staphylococcus epidermidis | ||
| NCTC11047 | Wild-type strain, laboratory culture collection | |
| Plasmid | ||
| pUC19 | High-copy-no. cloning vector, MCS, Ampr | Gibco BRL |
| pUC4-KIXX | Source of Tn5-derived nptII gene (Kmr) | Pharmacia |
| pBBR1MCS | Broad host range, IncP, -W, -Q, ColE1 and p15A compatible, containing pBluescript IIKS-lacZα-polylinker, Cmr | 15 |
| pKNG101 | oriR6K mobRK2 strAB sacBR, 6.8 kb, Smr | 10 |
| pZErO2.1 | Cloning vector Kmr | Invitrogen |
| pET28a(+) | E. coli overexpression vector for N-terminal His6-tagged proteins, Kmr | Novagen |
| pKAGb4(−) | Broad-host-range vector ori1600 carrying promoterless lacZ gene, Cmr | (M. S. Thomas) |
| pTB037 | 78 bp, encoding MshA N-terminal region in pZErO2.1 | This study |
| pETMshA | pET28a(+) expressing MshA as an N-terminal His6-tagged protein, Kmr | This study |
| pETMshB | pET28a(+) expressing MshB as an N-terminal His6-tagged protein, Kmr | This study |
| pBBRMshA | mshA of A. veronii bv. Sobria BC88 in pBBR1MCS, Cmr | This study |
| pBBRMshB | mshB of A. veronii bv. Sobria BC88 in pBBR1MCS, Cmr | This study |
| pBBRMshC | mshC of A. veronii bv. Sobria BC88 in pBBR1MCS, Cmr | This study |
| pBBRMshD | mshD of A. veronii bv. Sobria BC88 in pBBR1MCS, Cmr | This study |
| pBBRMshH | mshH of A. veronii bv. Sobria BC88 in pBBR1MCS, Cmr | This study |
| pBBRTapD | tapD of A. veronii bv. Sobria BC88 in pBBR1MCS, Cmr | This study |
| pKAG-PB | Promoter probe suicide vector pKAGb4 with BC88 mshB promoter region ligated upstream of the promoterless lacZ gene, Cmr | This study |
| pKAG-PA | Promoter probe suicide vector pKAGb4 with BC88 mshA promoter region ligated upstream of the promoterless lacZ gene, Cmr | This study |
| pKAG-PC | Promoter probe suicide vector pKAGb4 with BC88 mshC promoter region ligated upstream of the promoterless lacZ gene, Cmr | This study |
| pKAG-PD | Promoter probe suicide vector pKAGb4 with BC88 mshD promoter region ligated upstream of the promoterless lacZ gene, Cmr | This study |
| pKAG-PI | Promoter probe suicide vector pKAGb4 with BC88 mshI promoter region ligated upstream of the promoterless lacZ gene, Cmr | This study |
a
Amp, ampicillin; Cm, chloramphenicol; Km, kanamycin; Nal, nalidixic acid; Sm, streptomycin; MCS, multiple cloning site.