Table 4.
Kinetic parameters
| Enzyme | Substrate | Km (mM) | Vmax (mM min−1) | kcat(min−1) | kcat/Km (mM−1 · min−1) |
|---|---|---|---|---|---|
| AbpA | d-Xylulose-5-phosphate | 1.02 ± 0.08 | 0.16 ± 0.003 | 1,638.0 ± 306.5 | 1,639.9 ± 422.6 |
| AbpA | CTP | 1.76 ± 0.14 | 0.10 ± 0.005 | 1,065.3 ± 60.9 | 610.5 ± 84.6 |
| AbpA | dCTP | 2.85 ± 0.08 | 0.15 ± 0.01 | 1,602.6 ± 106.8 | 563.8 ± 53.3 |
| AbpB | CDP-d-xylulose | 1.53 ± 0.09 | 0.09 ± 0.01 | 486.8 ± 76.3 | 322.2 ± 68.8 |
| AbpB | NADH | 1.88 ± 0.17 | 0.035 ± 0.015 | 190.0 ± 81.2 | 101.5 ± 49.8 |
| AbpB | NADPH | 1.14 ± 0.11 | 0.23 ± 0.038 | 2,456.1 ± 396.9 | 2,192.1 ± 512.5 |