Fig 2.
The spontaneous deletion of wbkA is linked to the emergence of R mutants. (A) IS711 fingerprinting characterization of R mutants (the arrow indicates the 3.8-kb fragment absent in R mutants). (B) PCR detection of wbkA gene (BAB1_0553). (C) PCR detection of gmd gene (BAB1_0545). (D) PCR detection of manBO-Ag gene (BAB1_0560). (E) Western blot analysis with LPS antibodies. Strains 2308 Nalr and B10 are the B. abortus parental S strains of R mutants R6 and R7, respectively, and strain R6/c-wbkA is plasmid wbkA-pMM14 complemented R6 mutant (Table 1). S-LPS and R-LPS are the purified products used as controls.