Fig 3.

IS-mediated recombination is responsible for the loss of wbkA. (A) Genetic organization of the chromosomal scar left behind the excision. (B) Genetic organization of the closed-circular intermediate or CI carrying the copy of IS711 (in both panels A and B, hatched arrows indicate the crossover between the ISBm1 ORFs, and the primer [see the legend for Fig. 1] positions are depicted by small black arrows). (C and D) PCR detection of recombination intermediates in B. abortus recA and ISBm1-related mutants (note that scar-alt and CI-alt correspond to alternative recombination products). (E) PCR quantification of circular intermediates during growth (results are the average ± the standard error of triplicate measurements). (F) PCR quantification of chromosomal scars (results are the average ± the standard error of duplicate measurement from three independent experiments).