Table 2.
Primers used in this study
| Primera | Sequence (5′-3′) | Purpose | Source or reference |
|---|---|---|---|
| BAB1_0553Fa | TGGTATATCCGGGTGTCTCG | wbkA detection | This study |
| BAB1_0553R | AAGCGTCCGTGCATTTCTAT | This study | |
| BAB1_0545F | CCGAATTCTTGGAATGGAGA | gmd detection | This study |
| BAB1_0545R | CAGTCGCGTAATGAGTCCAA | This study | |
| BAB1_0560F | CTTATGCAATGGCTCCCAAT | manBO-Ag detection | This study |
| BAB1_0560R | CATGTTCGGCGATAAATGTG | This study | |
| BAB1_0545Fb | TGCGACTTTCTTCACGATTG | Detection of wbkA deletion | This study |
| BAB1_0558R | GATCTTGGTATCGGCCTGTC | This study | |
| BAB1_0557F | CGCTTTAATATCTCGCGTTCC | Detection of excised circle | This study |
| BAB1_0548R | GGTCCCATCGGCATATCTT | This study | |
| recA_F1 | TGTTATCCCTGTCGCCAAAT | To construct recA deletion mutant | This study |
| recA_R2 | CTTTCCGGCCCATAGATTTC | This study | |
| recA_F3 | GAAATCTATGGGCCGGAAAGGCTGGTCGATCTTGGTGTC | This study | |
| recA_R4 | ACCGCTTTCCTGTCCAGATA | This study | |
| BAB1_0546_F1 | AGTCCACCTTGACATGCACA | To construct a 150-bp deletion ISBm1-left | This study |
| BAB1_0546_R2 | CCATAGCGTTCGGGAACTT | This study | |
| BAB1_0547_F3 | AAGTTCCCGAACGCTATGGAAAAAGGGGATGGAGACGAT | This study | |
| BAB1_0547_R4 | ACGATTGCAGCCTCTTCCAC | This study | |
| BAB1_0557_F1 | CGACGAAGGCGTTTTACAG | To construct a 150-bp deletion ISBm1-rightb | This study |
| BAB1_0558_R4 | GATCTTGGTATCGGCCTGTC | This study | |
| BAB1_0553F-gwc | GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGCGAATTGGTGTCGAC | To complement the spontaneous ΔwbkA mutant | This study |
| BAB1_0553R-gw | GGGGACCACTTTGTACAAGAAAGCTGGGTCTTAATAGGTCATGAGCTTAGATT | This study | |
| 711u | CACAAGACTGCGTTGCCGACAGA | IS711 fingerprinting | 28 |
| 711d | CATATGATGGGACCAAACACCTAGGG | 28 | |
| scar_Fq | TTTGAAACGGTGCCCAAG | qPCR for chromosomal scar | This study |
| scar_Rq | ATAGACCCAGCGCGAAAAC | This study | |
| wbkA_Fq | TGCCGTCTCTCTACGAAGGT | qPCR for wbkA gene | This study |
| wbkA_Rq | TTCGGCTACGTTCAGAGGAT | This study |
a
F, forward; R, reverse.
b
These primers were used along with the primers BAB1_0546_R2 and BAB1_0547_F3 to construct the same deletion into ISBm1-right.
c
Sequences including att sites for BP clonase reaction are indicated in boldface.