Fig 5.
CglD domain structure and phenotypes. (A) Relevant features include type II signal sequence (SS), repeat elements 1 and 2, VWA domain (VWA) and C-terminal cysteine-rich domain. CglD amino acid substitutions and the ΔcglD deleted region (black bar) are shown for indicated mutants. (B) Colony edge morphologies of cglD strains in isogenic ΔpilQ background. Strains used were DK8615 (cglD+), DW1440 (cglD1), DW1461 (cglD2) and DW1433 (ΔcglD). Micrographs (10× objective) were taken after 1 day incubation on [1/2] CTT 1.5% agar supplemented with 2 mM CaCl2, except as noted.