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Addition of synthetic CglF peptide stimulates a cglF mutant in a TraA-independent manner. CglF synthetic peptide (20 μg) was mixed with nonmotile (A− S−) cglF mutants and incubated as shown. Strains used were DW1460 (ΔcglF), DW1405 (cglF1 traA::km) and the nonmotile donor strain was DK6204.
