Table 1.
Strains, plasmids, and primers used in this study
| Plasmid, strain, or primer | Description or relevant characteristic(s) | Source or reference |
|---|---|---|
| Plasmids | ||
| pYF27601 | Colicinogenic plasmid from Y. frederiksenii Y27601 | This study |
| pNKBOR | Suicide vector | 58 |
| pCR 2.1-TOPO | Commercial cloning vector | Invitrogen |
| pBAD-A | Commercial expression vector | Invitrogen |
| pDS1006 | pCR 2.1-TOPO carrying cfyA to cfyI from Y. frederiksenii Y27601 | This study |
| pDS1068 | pBAD-A carrying cfyA to cfyI from Y. frederiksenii Y27601 | This study |
| pDS1088 | pCR 2.1-TOPO carrying yiuBCR from Y. kristensenii Y276 | This study |
| pDS1082 | pCR 2.1-TOPO carrying yiuR from Y. kristensenii Y276 | This study |
| pDS1091 | pCR 2.1-TOPO carrying yiuR and tonB from Y. kristensenii Y276 | This study |
| Strains | ||
| E. coli DH5α pir | DH5α−derived strain expressing the R6K π protein | 58 |
| E. coli DH10B | Commercial cloning strain | 25 |
| E. coli TOP10F′ | Commercial expression strain | Invitrogen |
| PK11.1 to PK11.12 | 12 strains of E. coli DH10B containing Tn7 insertion in pYF27601 | This study |
| Primers | ||
| NKBORout3 | 5′-AACAAGCCAGGGATGTAACG-3′ | This study |
| NKBORout4 | 5′-GCAGGGCTTTATTGATTCCA-3′ | This study |
| colYF-XhoI-F | 5′-AGGACTCGAGATGACAGATTATAAAGATGTTGATCCG-3′ | This study |
| immYF | 5′-AGGACTCGAGATGGATATTAGATACTATATAAAAAATATA-3′ | This study |
| YE1459SD-F | 5′-ACCGAAATAAATGAGCCTATCCACTGAAT-3′ | This study |
| YE1461SD-F | 5′-ACCGAAATAAATGGCTAAGGCCTTTAGG-3′ | This study |
| YE1461-R | 5′-TTAGAAATCGTAGCTGGCGCCCAC-3′ | This study |
| infusionYE2222F | 5′-CTGGCGGCCGCTCGAGATGCAGCTAAATAAATTTTTCTTGGGTCGACGGC-3′ | This study |
| infusionYE2222R | 5′-AATTGGGCCCTCTAGATTAGTCCATTTCCGTCGTGCCGCCAATT-3′ | This study |