Table 2.
Primers used in this study
| Primer | Sequence (5′ to 3′)a | Use |
|---|---|---|
| Rmpyc | AGAGTGGCGTGAACATTCGG | expR restoration |
| SmndvA2 | TCCTTCTGTGACGAGATCG | expR restoration |
| ExpR.1 | AAAAAGCTTGCTTTTCGAGATAGACCTCG (HindIII) | expR deletion |
| ExpR.2 | CGTACAGTTTCTGGCTGGTACATGAACG | expR deletion |
| ExpR.3 | CGTTCATGTACCAGCCAGAAACTGTACGAGC | expR deletion |
| ExpR.4 | AAAGGATCCCGTGAACTTCTTCAGTTCGC (BamHI) | expR deletion |
| delexoY.1 | AAAGGATCCACCTCATAAGAGTTGTTGCC (BamHI) | exoY deletion |
| delexoY.2 | GGACATATTGCGTGTTTGCCATACCTCC | exoY deletion |
| delexoY.3 | GGAGGTATGGCAAACACGCAATATGTCC | exoY deletion |
| delexoY.4 | AAAGGATCC AATACCGTCAAATTGGGAGC (BamHI) | exoY deletion |
| exoX1 | AATAAGCTTGGACTTCATAGAGGTGACTC (HindIII) | exoX deletion |
| exoX2 | GCTCAGGAATTGAGGGTGCGAACATGGC | exoX deletion |
| exoX3 | GCCATGTTCGCACCCTCAATTCCTGAGCGGC | exoX deletion |
| exoX4 | AATGGATCCGAGCGTAGAGATCGTAATC (BamHI) | exoX deletion |
a
Restriction sites used for cloning (underlined) are given in parentheses after the sequence.