Fig 2.
Differential ability of the Δhfq mutant to form biofilm in vitro. (A and B) Growth of Y. pestis KIM6+ wild-type (black bars), Δhfq mutant (unfilled bars), and complemented mutant Δhfq (pCR4::hfq) (dark gray bars) strains in TMH medium (0.2% galactose, 1 μg/ml hemin) seeded at 103 bacteria/ml on polystyrene microtiter plates (A) and in borosilicate glass tubes (B). (C) The Δhfq mutant does not form rugose (wrinkled) colonies. Means and standard deviations of the results of 3 independent experiments are indicated for panels A and B. Data were analyzed using two-way ANOVA and a Bonferroni posttest. * indicates P < 0.05; **** indicates P < 0.001.