Table 1.
Bacterial strains and primers used in the study
| Bacterial strain or RT-qPCR primer | Forward primer 5′–3′ | Reverse primer 5′–3′ | Target gene (source or reference) |
|---|---|---|---|
| Bacterial strains | |||
| Actinomyces naeslundii (MG1) | GAGAAGAACTCCCTATCCATACC | CTTCATGGGTTGGGATTTCCT | Target is fimA gene, a fimbrial protein (this work) |
| Fusobacterium nucleatum (ATCC10953) | CTTATACATAGAGGACACAGAC | TTTCCAACAACATCTCCCTG | HprK gene (30) homologue of HPr kinase/phosphorylase (this work) |
| Lactobacillus casei (ATCC 334) | TGAATATTTACACGGTTCGGCA | GGGCCTTAGTTTCATCCGAC | Target is the phosphoglucomutase gene (10) (this work) |
| Streptococcus mitis (NCTC 12261, ATCC 49456) | CGTATCGGTCGTCTTGCT | TTGCATAACTGGATCTGTAAGGTC | Target is the glyceraldehyde-3-phosphate gene (this work) |
| Veillonella parvula (ATCC17745) | ACAGTTACTTTATAGTCTGTACC | TGGTCAATTGGCTAAACGTCAA | Target is the chaperone dnaK gene (29) (this work) |
| Aggregatibacter actinomycetemcomitans (HK1651) | ACGCAGACGATTGACTGAATTTAA | GATCTTCACAGCTATATGGCAGCTA | Target is lktC gene part of the leukotoxin operon lktBAC (54) |
| Porphyromonas gingivalis (ATCC 33277) | CCTACGTGTACGGACAGAGCTATA | AGGATCGCTCAGCGTAGCATT | Target is the Arg-gingipain gene (54) |
| RT-qPCR primers | |||
| Universal | CGCTAGTAATCGTGGATCAGAATG | TGTGACGGGCGGTGTGTA | 16S rRNA (79) |
| Actinomyces naeslundii (MG1) | ATCTCCAAGGTTCTGCACGACGAGTA | ATGTTGATGGTGATACCGCGCTGA | ANA_0022 (this work) |
| Aggregatibacter actinomycetemcomitans (HK1651) | AAGAACTTAACGCTTGGGCGATGC | TAACGCTTCTTGTGCAACACTGGC | AA00470 (this work) |
| Veillonella parvula (ATCC17745) | AAAGCCTTGGGCCATTCTCTGTTG | CCAAGGCCTCTTGTTCTTGCATCA | HMPREF1035_1004 (this work) |
| Porphyromonas gingivalis (ATCC 33277) | AGAAAGCCAGCCATTGTTGCATGG | TGTTCGGGACACCTGACTGTTCAT | PGN_0074 (this work) |