Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype and/or description | Reference or source |
|---|---|---|
| Strains | ||
| Y. pestis | ||
| KIM6+ | Wild type (pCD1-) | 14 |
| CDY319 | ΔrcsD::kan | 33 |
| CDY325 | ΔrcsB rcsD::kan | 33 |
| CDY326 | ΔrcsB::kan | 33 |
| CDY330 | ΔrcsA::rcsAYPSTB | 33 |
| CDY377 | ΔrcsD::kan (pCBD190) | 33 |
| CDY421 | ΔrcsB::kan ΔrcsA::rcsAYPSTB | 33 |
| CDY475 | KIM6+ (pCBD209) | This study |
| CDY497 | ΔhmsT::cat | This study |
| CDY567 | ΔlacZ::cat hmsH::lacZ | This study |
| CDY568 | ΔlacZ::cat hmsH::lacZ ΔrcsA::rcsAYPSTB | This study |
| CDY614 | ΔlacZ::cat hmsP::lacZ | This study |
| CDY615 | ΔlacZ::cat hmsP::lacZ ΔrcsA::rcsAYPSTB | This study |
| CDY622 | ΔlacZ | This study |
| CDY635 | ΔlacZ hmsT::lacZ | This study |
| CDY636 | ΔlacZ hmsT::lacZ (pCBD209) | This study |
| CDY637 | ΔlacZ hmsT::lacZ (pBAD/Myc-His) | This study |
| CDY638 | ΔlacZ hmsT::lacZ (pCBD178) | This study |
| CDY639 | ΔlacZ hmsT::lacZ (pCBD179) | This study |
| CDY641 | KIM6+ (pCBD253) | This study |
| CDY642 | KIM6+ (pCBD254) | This study |
| CDY981 | ΔhmsT::cat (pCBD26) | 32 |
| CDY982 | ΔrcsA::rcsAYPSTB (pCBD26) | This study |
| CDY983 | RcsAB* | This study |
| CDY984 | ΔrcsA::rcsAYPSTB RcsAB* | This study |
| CDY1071 | ΔrcsA::rcsAYPSTB ΔlacZ hmsT::lacZ | This study |
| SY743 | KIM6+ (pYC219) | This study |
| SY744 | KIM6+ (pYC220) | This study |
| Y. pseudotuberculosis | ||
| IP32953 | Wild type; serogroup O1 | 7 |
| CDY564 | ΔrcsA::rcsAYPE | 33 |
| CDY985 | IP32953(pCBD26) | This study |
| Plasmids | ||
| pBAD/Myc-His | Expression vector | Invitrogen |
| pCBD26 | hmsT in pCR2.1-TOPO | 31 |
| pCBD178 | rcsAYPE in pUC18 | 33 |
| pCBD179 | rcsAYPSTB in pUC18 | 33 |
| pCBD190 | rcsDYPSTB in PET32a | 33 |
| pCBD209 | rcsB in pBAD/Myc-His | This study |
| pCBD253 | rcsAYPSTB in pMal-4x | This study |
| pCBD254 | rcsAYPE in pMal-4x | This study |
| pCR2.1-TOPO | Cloning vector for PCR products | Invitrogen |
| pUC18 | Cloning vector | 35 |
| pMal-c4x | Expression vector | NEB |
| pYC219 | rcsAYPE-His6 in pUC18 | This study |
| pYC220 | rcsAYPSTB-His6 in pUC18 | This study |