Fig 2.

Accumulation of carboxysomes in cells over time, after transfer to growth medium with 1 mM DIC. Cells were initially cultivated in the presence of 50 mM DIC, harvested, washed, and resuspended in medium with 1 mM DIC. Depicted in transmission electron micrographs are cells cultivated in 50 mM DIC (A) and cells 3 h after resuspension in the absence (B) or presence (C) of 25 mg/liter of rifampin. For one cell, carboxysomes are indicated with arrows. (D) Frequencies of carboxysomes per cell area in electron micrographs. Cultures to which rifampin have been added are indicated as “Rif.”