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. 2012 Apr;194(8):2074–2081. doi: 10.1128/JB.06504-11

Table 1.

Primers and probes used to target T. crunogena conserved hypothetical genes

Locus tag Purpose Function Sequence (5′–3′)a Location on geneb (nt)
Tcr_1019 cDNA synthesis R primer ACGCGGTTAGATCCCATTG 206
qRT-PCR F primer AGAAAGCCGGCCGCTAAAA 130
R primer CCGGTTCTTCTTTTTCAGGTTGTTT 175
Probe FAM CCGGTTGCCAAACAG NFQ 150
Tcr_1315 cDNA synthesis R primer GGTATACGCCAGGTCATTGG 653
qRT-PCR F primer CCGTCGGGATTTTGAATGAAACC 427
R primer GGGTTACGCTCAACGCCATAA 468
Probe FAM ACGAACCACCAACTTT NFQ 452
Tcr_0466 cDNA synthesis R primer ACGGCACCATCTTTTGTTTC 541
qRT-PCR F primer GGGTTTGACCGCATGTATAACGA 343
R primer GATCGTAACGCCACCGAAAC 382
Probe FAM CTGCCGACAGATTTA NFQ 368
Tcr_2018 cDNA synthesis R primer ATATCGGCTTTTGGACAACG 1423
qRT-PCR F primer GCCGATATCGCTGGTGTAGAATTT 1075
R Primer GTTTTGCCAGGTTGAAGTCGTATT 1117
Probe FAM CCAAGCGCCATATTC NFQ 1099
16S (Tcr_R0053) cDNA synthesis R primer TTTATGAGATTCGCGCACTG 1206
qRT-PCR F primer CGAATATGCTCTACGGAGTAAAGGT 110
R primer CGCGGGCTCATCCTTTAG 157
Probe VIC CCCTCTCCTTGGAAGGT NFQ 136
a

FAM and VIC refer to the fluorescence tags, while NFQ refers to the major groove binder and nonfluorescent quencher (ABI, Carlsbad, CA).

b

Number of nucleotides at the 3′ end of the start codon of the gene.