Table 1.

Primers and probes used to target T. crunogena conserved hypothetical genes

Locus tag	Purpose	Function	Sequence (5′–3′)a	Location on geneb (nt)
Tcr_1019	cDNA synthesis	R primer	ACGCGGTTAGATCCCATTG	206
	qRT-PCR	F primer	AGAAAGCCGGCCGCTAAAA	130
		R primer	CCGGTTCTTCTTTTTCAGGTTGTTT	175
		Probe	FAM CCGGTTGCCAAACAG NFQ	150
Tcr_1315	cDNA synthesis	R primer	GGTATACGCCAGGTCATTGG	653
	qRT-PCR	F primer	CCGTCGGGATTTTGAATGAAACC	427
		R primer	GGGTTACGCTCAACGCCATAA	468
		Probe	FAM ACGAACCACCAACTTT NFQ	452
Tcr_0466	cDNA synthesis	R primer	ACGGCACCATCTTTTGTTTC	541
	qRT-PCR	F primer	GGGTTTGACCGCATGTATAACGA	343
		R primer	GATCGTAACGCCACCGAAAC	382
		Probe	FAM CTGCCGACAGATTTA NFQ	368
Tcr_2018	cDNA synthesis	R primer	ATATCGGCTTTTGGACAACG	1423
	qRT-PCR	F primer	GCCGATATCGCTGGTGTAGAATTT	1075
		R Primer	GTTTTGCCAGGTTGAAGTCGTATT	1117
		Probe	FAM CCAAGCGCCATATTC NFQ	1099
16S (Tcr_R0053)	cDNA synthesis	R primer	TTTATGAGATTCGCGCACTG	1206
	qRT-PCR	F primer	CGAATATGCTCTACGGAGTAAAGGT	110
		R primer	CGCGGGCTCATCCTTTAG	157
		Probe	VIC CCCTCTCCTTGGAAGGT NFQ	136

^aFAM and VIC refer to the fluorescence tags, while NFQ refers to the major groove binder and nonfluorescent quencher (ABI, Carlsbad, CA).

^bNumber of nucleotides at the 3′ end of the start codon of the gene.