Table 2.
Response of T. crunogena hypothetical gene transcription to changes in growth conditions
| Locus tag | Response to low DIC |
Response to low NH4+ |
Response to low PO4−x |
|||
|---|---|---|---|---|---|---|
| Fold increasea,b | ΔΔCT ± SD | Fold increasea,c | ΔΔCT ± SD | Fold increasea,d | ΔΔCT ± SD | |
| Tcr_1019 | 4.4 | −2.1 ± 0.2 | ||||
| Tcr_1315 | 6.7 | −2.8 ± 0.3 | ||||
| Tcr_0466 | 202 | −7.6 ± 1.0 | 0.77 | 0.3 ± 2.3 | ||
| Tcr_2018 | 7.0 | −2.8 ± 1.2 | 0.69 | 0.6 ± 1.4 | ||
a
qRT-PCR was carried out on RNA extracted from T. crunogena cells using 16S transcripts as the calibrator.
b
Fold increase is the frequency of transcription in low-DIC (high-NH4+, high-PO4−x) cells divided by the frequency in high-DIC (low-NH4+, high-PO4−x) cells.
c
Fold increase is the frequency of transcription in low-NH4+ (high-DIC, high-PO4) cells divided by the frequency in high-NH4+ (low-DIC, high-PO4−x) cells.
d
Fold increase is the frequency of transcription in low-PO4−x (high-DIC, high-NH4+) cells divided by the frequency in high-PO4−x (high-DIC, high-NH4+) cells.