Fig 5.
Measurement of growth inhibition due to overproduction of PomA/PomBΔL(Δ41–120). (A) The growth rate of each strain was measured, and representative examples are shown. NMB191 cells carrying the empty vector pBAD33 (■), pHFAB producing PomA/PomB (□), and pTSK37 producing the plug-deleted stator, PomA/PomBΔL(Δ41–120) (●), and with PomB-D24N (♦), with PomA-H136Y (♢), or with PomA-R247C (○) were grown in the presence of 0.2% (wt/vol) arabinose, and optical densities at 660 nm (OD660) were measured every hour. (B) Protein expression of the stator protein. PomA and PomB or PomBΔL(Δ41–120) expressed from pHFAB or pTSK37, respectively, were expressed in NMB191 cells. Each plasmid was induced with 0.2% (wt/vol) arabinose. PomA and PomB were detected by immunoblotting using anti-PomA and anti-PomB antibodies.