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Competitive indices for tonB mutant strains. Mixtures of the strains analyzed (0.1 ml) were injected s.c. in iron-overloaded animals as described in Materials and Methods. When animals showed signs of the disease, organs were removed and homogenized and bacterial cells plated on TSBS plus 1.5% agar (TSAS)–X-Gal plates. Bars represent the geometric mean CI value for each organ. Experiments were performed twice. ***, P < 0.001 based on one-way ANOVA with Bonferroni's posttest.
