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. 2001 Apr 17;98(9):5187–5192. doi: 10.1073/pnas.071050398

Figure 4.

Figure 4

PI3K activity in MCK-hLAR mice. Phosphotyrosine- (a), IRS-1- (b), or IRS-2-associated (c) PI3K activity in muscle of male mice, phosphotyrosine-associated PI3K activity in muscle of female mice (d), and IRS-1-associated PI3K activity in liver of female mice (e). Mice were fasted for 16–18 h, injected i.v. with saline or insulin (10 units/kg), and killed 3 min postinjection. PI3K activity was measured in phosphotyrosine, IRS-1, or IRS-2 immunoprecipitates of hind-limb muscles or liver as described in Materials and Methods. Results are mean ± SEM for three to five mice per group. *, difference from nontransgenic insulin-stimulated is P < 0.05. Similar results were seen in male and female mice.