Table 1.

Presentation of the 16SDPO-forward and 16SDPO-reverse primers together with the most common and aberrant sequence variants in their binding areas

Primer name and target organism	Sequenceb
16SDPO-forward	5′-AGAGTTTGATCMTGGCTCA-I-I-I-I-I-AACGCT-3′
Most bacteriaa	5′-AGAGTTTGATCMTGGCTCA-G-R-D-Y-D-AACGCT-3′
Atopobium spp.	5′------Y--------------I-I-I-I-I--------3′
Chlamydiales	5′----A-------T---T----I-I-I-I-I--------3′
Borrelia spp.	5′------------------T--I-I-I-I-I--------3′
Bifidobacteriales	5′-G----C----T---------I-I-I-I-I--------3′
Coxiella burnetii	5′-----------T---------I-I-I-I-I--------3′
16SDPO-reverse	5′-CGCGGCTGCTGGCA-I-I-I-A-I-TTRGC-3′
Most bacteriaa	3′-CGCGGCTGCTGGCA-C-R-D-A-D-TTRGC-5′
Chlamydiales	3′----A-----------I-I-I-A-I-------5′
Mycoplasma pneumoniae	3′-----A----------I-I-I-A-I-------5′
Ehrlichia spp.	3′----------------I-I-I-A-I---T---5′
Anaplasma phagocytophilum	3′----------------I-I-I-A-I---T---5′
Jonquetella anthropi	3′----------------I-I-I-A-I---T---5′

^aConsensus sequence based on the most commonly observed sequence variants.

^bBoldface letters indicate a locked nucleic acid. D is A, G, or T; M is A or C; R is A or G; Y is C or T; I is deoxyinosine.