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Intracellular distribution of AFB in lymphocytes on the modified Ziehl-Neelsen stain. Double labeling of AO (A and D, green) with CD3 (B, red) and CD20 (E, red) shows the intracellular location of AFB in lymphocytes (C and F). The nuclei are stained by Hoechst 33342 (blue). Scale bar, 5 μm.
