Table 1.
Comparison of techniques for detection of carbapenemase-producing A. baumannii isolates
| Isolated | Acquired carbapenemase(s) | MIC (μg/ml) |
Result of: |
Specific activity (mU/mg)a |
PCR resultc | Reference(s) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTX | CAZ | IPM | IPM+EDTA | MEM | DOR | Etest MBL | IPM/IPM+EDTA test (diameter −/+ EDTA) (mm) | Hodge test | IPM | MEM | ERT | ||||
| SLO | NDM-1 | >256 | >256 | >32 | 1 | >32 | >32 | + | + (6/25) | − | 5.1 | 0.6 | 1.4 | + | This study |
| All | NDM-1 | >256 | >256 | >32 | <1 | >32 | >32 | + | + (6/24) | − | 5.9 | 0.9 | 1.4 | + | 9 |
| Ora-1 | NDM-1 | >256 | >256 | >32 | <1 | >32 | >32 | + | + (6/22) | − | 6.4 | 0.9 | 1.1 | + | This study |
| StN | NDM-1 | >256 | >256 | >32 | <1 | >32 | >32 | + | + (6/23) | − | 6.3 | 0.8 | 1.2 | + | This study |
| Gen | NDM-1, OXA-23 | >256 | >256 | >32 | 3 | >32 | >32 | + | + (6/18) | − | 7.2 | 1.1 | 1.5 | + | 20 |
| ML | NDM-2 | >256 | >256 | >32 | <1 | >32 | >32 | + | + (6/23) | − | 4.7 | 0.9 | 1.3 | + | 10 |
| 124 | NDM-2 | >256 | >256 | >32 | <1 | >32 | >32 | + | + (6/23) | − | 4.5 | 0.7 | 1.3 | + | This study |
| IMP | IMP-4 | >256 | >256 | 4 | <1 | 8 | 4 | + | −/+ (14/20) | − | 718.1 | 95.6 | 65.2 | − | 4 |
| A154 | VIM-4 | >256 | >256 | 32 | <1 | 32 | 32 | + | + (8/25) | + | 279.8 | 45.2 | 33.3 | − | 6 |
| SIM | SIM-1 | >256 | >256 | 3 | <1 | 4 | 3 | −/+e | −/+ (21/29) | +b | 58.3 | 12.6 | 23.2 | − | 15 |
| 35 | OXA-23 | >256 | >256 | 32 | 4 | 32 | 24 | −/+ | −/+ (9/17) | − | 2.8 | 1.1 | 0.8 | − | This study |
| Acb2 | OXA-23 | >256 | >256 | 32 | 4 | 32 | 24 | −/+ | −/+ (11/20) | − | 2.1 | 1.1 | 0.8 | − | 2 |
| Acb13 | OXA-23 | >256 | >256 | 24 | 4 | 24 | 16 | −/+ | −/+ (11/18) | − | 1.9 | 1.2 | 0.9 | − | 2 |
| LAH | OXA-40 | >256 | 128 | 32 | 12 | >32 | 32 | −/+ | −/+ (7/18) | +b | 2.1 | 0.5 | 0.5 | − | This study |
| MUZ | OXA-40 | >256 | 256 | 32 | 12 | >32 | 32 | −/+ | −/+ (7/16) | +b | 1.6 | 0.6 | 0.4 | − | This study |
| Acb19 | OXA-58 | >256 | >256 | 4 | 2 | 4 | 4 | − | − (14/18) | − | 3.1 | 1.2 | 1.5 | − | 2 |
| MAY | OXA-58 | 8 | 2 | 3 | 2 | 2 | 1 | − | − (15/17) | − | 2.9 | 1.1 | 0.6 | − | This study |
| MAD | OXA-58 | 256 | 256 | 24 | 16 | 24 | 24 | − | − (11/15) | +b | 3.0 | 1.2 | 0.6 | − | 19 |
| AP | GES-14 | >256 | >256 | 24 | 8 | 16 | 8 | − | − (11/17) | +b | 7.5 | 1.4 | 1.1 | − | 1 |
a
Standard deviations were within 10% of the means.
b
Weakly positive.
c
PCR was performed under standard conditions with NDM-For and NDM-Rev.
d
All isolates are A. baumannii except isolate A154, which belongs to genomospecies 16.
e
−/+, indeterminate result.