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Specificity of the assay with full-length positive-strand RNA. A negative-strand assay was performed on 107, 106, 105, 104, and 103 (lanes 1, 2, 3, 5, and 6, respectively) copies of HEVT1FG positive-sense synthetic RNA in the presence of 1 μg of total cellular RNA. Nuclease-free water served as the negative control (lane 7).
