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Negative-strand assay in the presence of positive-sense RNA: a negative-strand assay was performed on 105, 104, 103, 102, and 10 (lanes 1, 2, 3, 5, and 6, respectively) copies of negative-sense synthetic RNA in the presence of 105 copies of positive-sense synthetic RNA and 1 μg of total cellular RNA. Nuclease-free water served as the negative control (lane 7).
