Fig 6.

Screening of HEV RNA-positive stool samples from acute hepatitis E cases. Negative-strand detection was carried out on RNA isolated as follows: lane 1, stool sample 1 (539 copies of HEV positive-sense genomic RNA/reaction); lane 2, stool sample 2 (1 × 10⁵ copies); lane 3, negative control; lane 4, stool sample 3 (1 × 10⁵ copies); lane 5, stool sample 4 (619 copies); lane 6, negative control; lane 7, stool sample 5 (9,591 copies); lane 8, stool sample 6 (1 × 10⁵ copies); lane 9, 100-bp ladder; lane 10, purified virus stock 1 (1.4 × 10⁴ copies); lane 11, virus stock 2 (2,653 copies); lane 12, virus stock 3 (1,782 copies).