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Sequences and locations of primers
| Primer | Sequencea | Position (nt) |
|---|---|---|
| HEVF1 | 5′TGAGAATGATTTCTCTGAGTTTG3′ | 4398–4420 |
| HEVF2 | 5′ATACCGTCTGGAACATGGC3′ | 4604–4622 |
| HEVR1 | 5′ATGTTATTCATTCCACCCG3′ | 5116–5098 |
| HEVR2 | 5′AGCATCCCAATCAGGTTATG3′ | 5018–4999 |
| HEVTagF1 | 5′CGGTCATGGTGGCGAATAATGAGAATGATTTCTCTGAGTTTG3′ | 4398–4420 |
| HEVTagR1 | 5′CGGTCATGGTGGCGAATAAATGTTATTCATTCCACCCG3′ | 5116–5098 |
| Tag | 5′CGGTCATGGTGGCGAATAA3′ |
Underlined sequences in HEVTagF1 and HEVTag R1 represent non-HEV sequences.
