Table 1.
Major and minimal epitopes of HRP-specific MAbs, occurrence in P. falciparum field isolates, and heat stability
| MAb | Major epitope(s) | Minimum epitopeb |
Reactivity before and after incubation at 37°Cc |
||||
|---|---|---|---|---|---|---|---|
| Epitope | Prevalence in isolates tested (%) | Avg frequency | Day 0 | 30 days | 90 days | ||
| 3A4a | DAHHAHHA | AHHAHHA | 448/448 (100) | 15 | +++++ | +++ | − |
| 2G12-1C12a | DAHHAADAHH | DAHHAADAHH | 448/448 (100) | 10 | +++++ | +++++ | − |
| DAHHVADAHH | |||||||
| 1E1-A9a | YAHHAHHA | AHHAHHV | 445/448 (99.3) | 3 | +++++ | +++ | − |
| DAHHAHHV | |||||||
| 4A5a | Conformational | Conformational | NA | NA | +++++ | ++++ | − |
| A6-4 | HATDAHHAAD | HATDAHH | 448/448 (100) | 5 | +++++ | ++ | − |
| HATDAHHAAA | |||||||
| C1-13 | AHHAADAHHA | AHHAADAHH | 448/448 (100) | 18 | +++++ | +++++ | +++ |
| N7 | DAHHAADAHHA | DAHHAADAHHA | 448/448 (100) | 10 | +++++ | +++ | − |
| PTL-3 | DAHHAHHA | YAHHAHHA | 439/448 (97.9) | 3 | +++++ | +++++ | +++ |
| YAHHAHHA | |||||||
| S2-5 | AHHAADAHH | AHHASDAHHA | 367/448 (82) | 1 | +++++ | − | − |
| AHHASDAHH | |||||||
| TC-10 | TDAHHAADAHHAADA | TDAHHAADAHHAADA | 327/448 (72.9) | 1 | ND | ND | ND |
| C2-3 | AHHAADAHH | HAHHAHHAADAHH | 123/448 (27.4) | 0 | +++++ | ++ | − |
| Genway | AAYAHHAHHAAY | AYAHHAHHAAY | 3/448 (0.01) | 0 | ND | ND | ND |
a
Epitope mapping previously published (8).
b
NA, not applicable.
c
Signal strength was graded on an arbitrary scale from +++++ (maximum) to + (just visible) to − (absent). ND, not determined.