Table 4.
Quantification of EBV DNA loads of QCMD 2009 proficiency panel specimens
| QCMD 2009 specimen no. | VL result (log10 copies/ml)a |
Difference from expected VL result (Δlog10 copies/ml) |
|||||
|---|---|---|---|---|---|---|---|
| Expected | Lab PCR | Artus PCR | Argene PCR | Lab PCR | Artus PCR | Argene PCR | |
| 1 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
| 2 | 2.425 | 2.512 | 2.000 | 2.989 | 0.087 | −0.425 | 0.564 |
| 3 | 2.719 | 2.845 | 2.000 | 2.544 | 0.126 | −0.719 | −0.175 |
| 4 | 3.919 | 4.145 | 3.653 | 3.803 | 0.226 | −0.266 | −0.116 |
| 5 | 4.218 | 4.390 | 3.309 | 3.922 | 0.172 | −0.909 | −0.296 |
| 6 | 4.431 | 4.512 | 4.525 | 4.290 | 0.081 | 0.094 | −0.141 |
| 7 | 5.199 | 5.279 | 4.813 | 4.942 | 0.080 | −0.386 | −0.257 |
| 8 | 2.733 | 3.051 | 2.000 | 2.398 | 0.318 | −0.733 | −0.335 |
| 9 | 3.412 | 3.641 | 3.544 | 3.051 | 0.229 | 0.132 | −0.361 |
| 10 | 3.315 | 3.577 | 2.699 | 2.813 | 0.262 | −0.616 | −0.502 |
a
Lab PCR, laboratory-developed PCR (on a LightCycler amplification platform); Artus PCR, Qiagen artus EBV RG PCR kit (on a Rotor-Gene amplification platform); Argene PCR, Argene EBV R-gene kit (on a Rotor-Gene amplification platform). The Spearman correlation coefficients with regard to the Expected value (P ≤ 0.006) were 0.999 for the Lab PCR, 0.951 for the Artus PCR, and 0.915 for the Argene PCR.